| 出品公司: | BD Biosciences |
|---|---|
| 载体名称: | pAcGHLT C |
| 质粒类型: | 昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 8753 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pAcGHLTC载体基本信息
pAcGHLTC载体图谱质粒图谱和多克隆位点信息
pAcGHLTC载体简介
pAcGHLTC载体序列
LOCUS pAcGHLT C 8753 bp ds-DNA circular SYN 19-APR-2014
DEFINITION Baculovirus transfer vector for expressing proteins with an
N-terminal GST-6xHis-PKA-thrombin cassette. For other reading
frames, use pAcGHLT A or pAcGHLT B.
ACCESSION .
VERSION .
KEYWORDS pAcGHLT C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8753)
AUTHORS BD Biosciences
TITLE Direct Submission
JOURNAL Exported Thursday, February 6, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8753
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
misc_recomb 1269..2096
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 1269..1393
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(1431..2036)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
promoter 2100..2191
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 2206..2859
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/note="GST"
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 2875..2892
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 2899..2916
/codon_start=1
/product="recognition site for phosphorylation by protein
kinase A"
/note="PKA site"
/translation="RRASVA"
CDS 2926..2943
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
misc_feature 2958..3025
/note="MCS"
/note="multiple cloning site"
misc_recomb 3045..4503
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(3162..4503)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin 5876..6331
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6613..6717
/gene="bla"
/note="AmpR promoter"
CDS 6718..7578
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7749..8337
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatccgat gggacatcat
2881 catcatcatc acggaaggag aagggccagt gttgcggcgg gaattttggt ccctcgtgga
2941 agcccaggac tcgatggcat atgctcgagg aattcaggcc tccatgggag ctcgcggccg
3001 cctgcagggt acccccggga gatctgtacc gactctgctg aagaggagga aattctcctt
3061 gaagtttccc tggtgttcaa agtaaaggag tttgcaccag acgcacctct gttcactggt
3121 ccggcgtatt aaaacacgat acattgttat tagtacattt attaagcgct agattctgtg
3181 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
3241 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
3301 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
3361 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
3421 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
3481 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
3541 tacaattgac tcgacgtaaa cacgttaaat aaagcttgga catatttaac atcgggcgtg
3601 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
3661 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
3721 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt
3781 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc
3841 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa
3901 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc
3961 ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca
4021 actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt
4081 ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga
4141 ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt
4201 ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt
4261 tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc
4321 acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa
4381 ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg
4441 ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc
4501 ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg
4561 agacacttcg ctgtcgtcga cgtacatgta tgctttgttg tcaaaaacgt cgttggcaag
4621 ctttaaaata tttaaaagaa catctctgtt cagcaccact gtgttgtcgt aaatgttgtt
4681 tttgataatt tgcgcttccg cagtatcgac acgttcaaaa aattgatgcg catcaatttt
4741 gttgttccta ttattgaata aataagattg tacagattca tatctacgat tcgtcatggc
4801 caccacaaat gctacgctgc aaacgctggt acaattttac gaaaactgca aaaacgtcaa
4861 aactcggtat aaaataatca acgggcgctt tggcaaaata tctattttat cgcacaagcc
4921 cactagcaaa ttgtatttgc agaaaacaat ttcggcgcac aattttaacg ctgacgaaat
4981 aaaagttcac cagttaatga gcgaccaccc aaattttata aaaatctatt ttaatcacgg
5041 ttccatcaac aaccaagtga tcgtgatgga ctacattgac tgtcccgatt tatttgaaac
5101 actacaaatt aaaggcgagc tttcgtacca acttgttagc aatattatta gacagctgtg
5161 tgaagcgctc aacgatttgc acaagcacaa tttcatacac aacgacataa aactcgaaaa
5221 tgtcttatat ttcgaagcac ttgatcgcgt gtatgtttgc gattacggat tgtgcaaaca
5281 cgaaaactca cttagcgtgc acgacggcac gttggagtat tttagtccgg aaaaaattcg
5341 acacacaact atgcacgttt cgtttgactg gtacgcggcg tgttaacata caagttgcta
5401 accggcggcc gacacccatt tgaaaaaagc gaagacgaaa tgttggactt gaatagcatg
5461 aagcgtcgtc agcaatacaa tgacattggc gttttaaaac acgttcgtaa cgttaacgct
5521 cgtgactttg tgtactgcct aacaagatac aacatagatt gtagactcac aaattacaaa
5581 caaattataa aacatgagtt tttgtcgtaa aaatgccact tgttttacga gtagaattag
5641 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
5701 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
5761 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
5821 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
5881 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
5941 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
6001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
6061 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
6121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
6181 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
6241 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
6301 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
6361 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
6421 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
6481 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
6541 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
6601 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
6661 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
6721 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
6781 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
6841 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
6901 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
6961 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
7021 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
7081 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
7141 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
7201 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
7261 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
7321 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
7381 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
7441 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
7501 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
7561 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
7621 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
7681 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
7741 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
7801 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
7861 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
7921 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
7981 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
8041 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
8101 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
8161 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
8221 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
8281 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
8341 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
8401 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
8461 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
8521 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
8581 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
8641 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
8701 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga att
//
